xepA [2019-11-11 15:09:01]

PBSX prophage lytic exoenzyme

Locus: BSU_12780

Isoelectric point: 5.42

Molecular weight: 30.26 kDa

Protein length: 279 aa

Gene length: 840 bp

Function: phage release

Product: PBSX prophage lytic exoenzyme

Essential: no

Synonyms: xkdY

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Cell envelope and cell division → Cell wall degradation/ turnover → Autolysis
• Prophages and mobile genetic elements → Prophages → PBSX prophage
• Groups of genes → Secreted proteins

Gene

Coordinates: 1,345,839 1,346,678

The protein

Paralogous protein(s)

• YqxG
• YomS: 38% identity to the C-terminal part of XepA

Localization

• extracellular (no signal peptide) PubMed

Expression and Regulation

Operons

• Genes: xtmA-xtmB-xkdE-xkdF-xkdG-xkdH-xkdI-xkdJ-xkdK-xkdM-xkdN-xkzB-xkdO-xkdP-xkdQ-xkdR-xkdS-xkdT-xkdU-xkzA-xkdV-xkdW-xkdX-xepA-xhlA-xhlB-xlyA
  Description: PubMed

  Sigma factors

  • Xpf: sigma factor, PubMed, in Xpf regulon

  Regulation

  • expression during spore germination is strongly reduced under conditions of osmotic stress PubMed

Biological materials

Mutant

• BKE12780 (xepA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTTCATCCTCCTTTGAT, downstream forward: _UP4_TAATAGTCTCGGCCCTCGGA
• BKK12780 (xepA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTTCATCCTCCTTTGAT, downstream forward: _UP4_TAATAGTCTCGGCCCTCGGA

References

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